PCR Techniques and Their Clinical Applications
نویسندگان
چکیده
Kary B. Mullis developed a revolutionary method name polymerase chain reaction (PCR) in 1983, which can synthesize new strand of DNA complementary to the template and produce billions copies fragment only few hours. Denaturation, annealing, extension are three primary steps involved PCR process, generally requires thermocyclers, template, pair primers, Taq polymerase, nucleotides, buffers, etc. With development PCR, from traditional quantitative next digital has become powerful tool life sciences medicine. Applications techniques for infectious diseases include specific or broad-spectrum pathogen detection, assessment surveillance emerging infections, early detection biological threat agents, antimicrobial resistance analysis. genetic prenatal diagnosis screening neonatal diseases. cancer research tumor-related gene detection. This chapter aimed discuss about different types techniques, including etc., their applications rapid mutation screen diseases, inherited cancer, other
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2023
ISSN: ['1520-4995', '1943-295X', '0006-2960']
DOI: https://doi.org/10.5772/intechopen.110220